the p414 peptide  (Genosphere)

Journal: Cell Reports Methods

Article Title: B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles

doi: 10.1016/j.crmeth.2023.100624

Figure Lengend Snippet: Membrane interaction of peptides followed by 1D- 1 H-NMR spectroscopy (A and B) NMR amide spectra upon titration of the P233 (A) and P414 (B) peptides with POPC:POPG:cholesterol 7:2:1 SUV. The lipid concentrations range from 0 (black) to 10 mM (dark yellow). The concentrations of the P233 and P414 peptides are 15 μM and 5 μM, respectively. The spectrum of lipid vesicles in the absence of peptides at the highest lipid concentration used is represented as a dashed line (yellow).

Article Snippet: The P414 peptide , Genosphere Biotechnologies , P414.

Techniques: Membrane, Structural Proteomics, Titration, Concentration Assay

Journal: Cell Reports Methods

Article Title: B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles

doi: 10.1016/j.crmeth.2023.100624

Figure Lengend Snippet: Membrane partitioning of the P233 and P414 peptides followed by 1D- 1 H NMR, tryptophan fluorescence, and far-UV CD (A–D) The experiments were performed in the presence of POPC:POPG:chol 7:2:1 SUV. Data for P233 and P414 as a function of lipid concentration are displayed in magenta and blue, respectively. Membrane partitioning followed by 1D- 1 H NMR is represented on a linear scale (A) and on a logarithmic scale (B). The ratio of tryptophan fluorescence intensity (320 nm/370 nm) (C) and the ellipticity at 222 ± 2 nm in the far-UV region (D) as a function of lipid concentration are shown. Error bars in (A and B) are calculated from the standard deviation of noise in 1D-NMR spectra. The signal loss in (A) and (B) of the tryptophan indol proton (open circles) followed by NMR is similar to that of the amide envelope (closed circles). Data in (C and D) are mean ± SEM.

Article Snippet: The P414 peptide , Genosphere Biotechnologies , P414.

Techniques: Membrane, Fluorescence, Concentration Assay, Standard Deviation

Journal: Cell Reports Methods

Article Title: B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles

doi: 10.1016/j.crmeth.2023.100624

Figure Lengend Snippet:

Article Snippet: The P414 peptide , Genosphere Biotechnologies , P414.

Techniques: Recombinant, Translocation Assay, Software